Integrated strategy of CRISPR-Cas9 gene editing and small RNA RhyB regulation in Enterobacter aerogenes: A novel protocol for improving biohydrogen production

Dark fermentation is considered as one of the most practical biological hydrogen production methods. However, current productivity and yield are still not economically viable for industrial applications. This biological process must be improved through multiple strategies, of which screening for more effective microbial strains is an important aspect. Here, based on the hydrogen production pathway of