Characteristic analysis of discontinuous function modelling-based nano-biosensor and its detection for hyaluronidase

This study draws attention to the characteristics of a nano-biosensor based on a discontinuous function algorithm and its application in hyaluronidase (HAase) detection. The discontinuous algorithm model is introduced in the construction of sensor. AuNPs nanoparticles and nanocomposite materials of Nation-Ru(bpy)32+-AuNPs and Nation-Ru(bpy)32+-AuNPs-TTX are prepared for chemical modification of the electrode. Finally, a new nano-electrochemical immunosensor is successfully constructed. Twenty ICR male mice aged 6-8 months were selected as research subjects. They were randomly divided into control group and observation group, with their sperm protein extracted for Western blot and enzyme activity detection. The results show that AuNPs nanomaterials have good biocompatibility and conductivity, and demonstrate good sensitivity in the detection process. The enzyme detection results show that the enzyme activity of the observation group is higher than that of control group, indicating that the prepared sensor system has relatively high accuracy, with the detection being simple, more sensitive, and quicker.