Polydopamine on Copper-Doped Cerium Dioxide Nanosheets as Peroxidase Mimics for the Intelligent Detection of Cholesterol

The catalytic ability of nanozymes has become an enzymology hotspot in the field of application. Most nanozymes were characterized to simultaneously have oxidase-like and peroxidase-like activities, but the practical application often focuses on certain activity; other complex activities may cause interference. The peroxidase-like activity (POD-like activity) of nanozymes have been widely used in the colorimetric detection of H₂O₂ or substances producing H₂O₂ as an intermediate, such as the detection of small biological molecules with the oxidative reaction of a chromogenic reagent in the presence of POD-like nanozymes. In this work, we used polydopamine (PDA) as the surface coating of Cu-CeO₂ nanosheets (PDA@ Cu-CeO₂), which enhanced peroxidase-like activity while inhibiting their oxidase-like activity, providing a feasible method for the sensitive determination of cholesterol by integrating visual colorimetric detection and a smartphone application as a readout. The absorbance intensity and RGB values displayed a linear range on cholesterol from 0.05 to 1.2 mM with the LOD (limit of detection) of 42.7 and 99.4 μM. In addition, the method is expected to apply in detecting cholesterol in human serum with acceptable accuracy.