适体
化学
生物传感器
检出限
DNA
分析物
组合化学
复式(建筑)
杂交探针
靶蛋白
分子信标
纳米技术
生物物理学
色谱法
生物化学
分子生物学
寡核苷酸
基因
生物
材料科学
作者
Pinghua Ling,Linyu Wang,Shan Cheng,Xianping Gao,Xinyu Sun,Feng Gao
标识
DOI:10.1016/j.aca.2022.339675
摘要
Herein, a cost-effective, simple and sensitive electrochemical sensing platform was established based on aptamer - target recognition and target-triggering signal amplification strategy for protein detection. Due to the high affinity between the aptamer and target, the assistant DNA1 (a1) could release from a1-aptamer duplex and trigger the following DNA circuits. The strand displacement and branch migration reaction brought assistant DNA3 (a3) released. Eventually, a large number of duplex structures of a3-Hairpin DNA3 were formed on the surface of electrode. Consequently, the capture DNA on the surface of platinum nanoparticles could hybridize with the unfolded DNA fragment of Hairpin DNA3 on the sensor surface, resulting in the electrochemical signal readout of H2O2 reduction. Using thrombin as a model target, under the optimal conditions, this method exhibited a linear detection range from 0.5 pM to 300 nM with a detection limit of 0.17 pM. The proposed detection strategy was enzyme-free and exhibited good selectivity and sensitivity for a variety of protein targets detection by using corresponding DNA-based affinity probes, which makes it possible to apply the sensor for sensitivity detection of analytes in bioassays.
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