Bacillus subtilis chassis in biomanufacturing 4.0

Abstract Synthetic biology, an emerging research field, can promote biomanufacturing 4.0 by offering various efficient chassis. Engineering Bacillus subtilis, a prominent workhorse in industrial biotechnology, through synthetic biology approaches may be a disruptive innovation. Diverse chassis forms are envisaged to deal with challenges in metabolic complexity, metabolic burden, and systems robustness. Thus, advancements in chassis engineering, a synthetic biology strategy for genome‐reduced cell factories, cell‐free systems, and synthetic microbial consortia would be a driving force facilitating microbial production. In this review article, we summarized chassis engineering categories and applications for B. subtilis. Prospects and challenges for chassis engineering in B. subtilis were also discussed. © 2022 Society of Chemical Industry (SCI).