神经肽
增食欲素
体内
体感系统
神经科学
生物
内生
食欲素-A
食欲素受体
细胞生物学
化学
受体
内分泌学
生物化学
生物技术
作者
Loïc Duffet,Seher Kosar,Mariangela Panniello,Bianca Viberti,Edward F. Bracey,Anna D Zych,Arthur Radoux-Mergault,Xuehan Zhou,Jan Dernič,Luca Ravotto,Yuan-Chen Tsai,Marta Figueiredo,Shiva K. Tyagarajan,Bruno Weber,Miriam Stoeber,Nadine Gogolla,Markus H. Schmidt,Antoine Adamantidis,Tommaso Fellin,Denis Burdakov,Tommaso Patriarchi
出处
期刊:Nature Methods
[Springer Nature]
日期:2022-02-01
卷期号:19 (2): 231-241
被引量:49
标识
DOI:10.1038/s41592-021-01390-2
摘要
Orexins (also called hypocretins) are hypothalamic neuropeptides that carry out essential functions in the central nervous system; however, little is known about their release and range of action in vivo owing to the limited resolution of current detection technologies. Here we developed a genetically encoded orexin sensor (OxLight1) based on the engineering of circularly permutated green fluorescent protein into the human type-2 orexin receptor. In mice OxLight1 detects optogenetically evoked release of endogenous orexins in vivo with high sensitivity. Photometry recordings of OxLight1 in mice show rapid orexin release associated with spontaneous running behavior, acute stress and sleep-to-wake transitions in different brain areas. Moreover, two-photon imaging of OxLight1 reveals orexin release in layer 2/3 of the mouse somatosensory cortex during emergence from anesthesia. Thus, OxLight1 enables sensitive and direct optical detection of orexin neuropeptides with high spatiotemporal resolution in living animals.
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