免疫分析
检出限
材料科学
黑磷
纳米技术
基质(水族馆)
胶体金
生物传感器
色谱法
化学
纳米颗粒
光电子学
抗体
免疫学
地质学
海洋学
生物
作者
Xiuting Li,Jiali Ma,Yongling Zhang,Lanxin Xu,Chenjie Gu,Guodong Wei,Xiaoling Zhang,Tao Jiang,Jun Zhou
标识
DOI:10.1016/j.bios.2022.114148
摘要
It is tremendously desirable for the timely and effective detection of cancer to facilitate the ultra-highly sensitive monitoring of tumor marker in clinical serum sample. In this study, an electromagnetic and chemical synergistically enabled recyclable immunoassay based on surface-enhanced Raman scattering (SERS) was proposed by realizing the anisotropic growth of sea-urchin-like gold nanoflowers (Au NFs) on two-dimensional red phosphorus (RP) nanoplates. Besides the achieved enhancement factor as high as 2.24 × 106, it was found that the photocatalytic and SERS activities were kept at a high level for the hybrid substrate of RP/Au NFs throughout 7 cycles of immunoassay. In combination with a non-metallic immunoprobe, the limit of detection was drove to 7.41 × 10-5 IU·mL-1 for cancer antigen 19-9. The comparative experiments of nonspecific monitoring verified the promising selectivity of this strategy. Considering the intriguing features of high sensitivity, recyclability, and specificity, the proposed multifunctional RP/Au NFs exhibited its superior role in the early detection of cancer and can be adapted for point-of-care diagnosis.
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