Breeding of high-yield tannase strain from Aspergillus niger by N~+ implantation

Using Aspergillus niger as original strain,mutated strain Aspergillus niger ANO2 was obtained by N+ implantation.The results show that the activity of tannase raised from 442.3 U/mL to 670.2 U/mL,which was 51.4% higher than the initial level.Through 10 generations succession cultivation,the enzyme producing capability of Aspergillus niger ANO2 tended to be stable,so it is an ideal tannase strain.