The experimental research of the influence of ~(59) Fe's metabolism of the rat of deficient spleen syndrome by replenishing spleen method

Objective:On basement of model of rat' deficient spleen syndrome,these are the dynamic observation studies of?~(59) Fe's obsorbed metabolism and /or transported metabolism course of the rat of deficient spleen syndrome and replenishing spleen method by the application of radioactivity label technology in pharmacokinetics.The experiment studies the relation of the spleen being the transferring owner or the spleen losing the transferring owner with Fe's metabolism,researches it's biology meaning of spleen being the owner of transmission,researches the science intension of important traditional Chinese medicine theory of spleen being the owner of transmission and the spleen serving as basement of the day after tomorrow,and provides the experiment basis of spleen merit ability and/or essece of deficient spleen syndrome.Methods:40 male SD rats were randomly divided into 4 groups:normal group,normal replenishing spleen group,deficient spleen group,deficient spleen-replenishing spleen group.According to the requirement of the animal model standardizes most of rats were made the pattern.4 goups were once iffigated shui xuanye of?~(59) FeCl_3 0.2ml/220g/a rat.At the same time,according to weight of a person and a rat,2 groups of normal replenishing spleen group and deficient spleen-replenishing spleen group were given 1.5m±medicinal ingredients liquid of 1:1 four-man decoction,the others were given 1.5m±physiological saline,once a day.Respectively at 0.5h,1h,1.5h,3h,6h,12h and 24h,blood 0.2ml of all rats were taken from rat's tail,measured its radioactivity?~(59) Fe with γ-radiation immunity counter,to observe changes of pharmacokinetics of?~(59) Fe passing through rat's stomach and bowel way into the whole body blood-system.Results:The experiment exists the clear difference for changes of radioactivity?~(59) Fe pharmacokinetics in the states of deficient spleen and replenishing spleen to absorb?~(59) FeCl_3 from rat's stomach and bowel way into the whole body blood-system.Replenishing spleen method have promoted the absorb to?~(59) FeCl_3.Conclusions:The rat of deficient spleen syndrome has reduced the bioavailability of?~(59) FeCl_3 and made it the lower lever to absorb of?~(59) FeCl_3.On the contrary,Replenishing spleen method has promoted to absorb?~(59)(FeCl_3).from rat's stomach and bowel way into the whole body blood-system.