A Study of Preliminary Purification and Scavenging Free Radical Activity of Polysaccharide of Toona sinensis Roem

In this experiment,the polysaccharide was extracted and purified from Toona sinensis Roem.We measured and purified the number of the polysaccharide protein,made an anti-oxide experiment for water-soluble polysaccharide and the purified polysaccharide,and measured the effect of eliminate of free radical for the different density of polysaccharide.The result shows that the composition of the polysaccharide was Xyl,Ara,Rha,Gal,Glc,Gal-A,and Man,and proportion of mol:1.0 ∶2.8 ∶2.6 ∶2.6 ∶2.0 ∶7.6 ∶1.6.A result obtained from anti-oxide experiment shows that different density of water-soluble polysaccharide and purified polysaccharide can eliminate free radical·OH and anions.Furthermore,the effect of purified polysaccharide is beyond of water-soluble polysaccharide.The water-soluble polysaccharide can eliminate R and the purified polysaccharide can not eliminate it.