Expression and polyclonal antibodies preparation of porcine epidemic diarrhea virus truncate S1 protein

The aim of this study was to express the truncated S1 gene(named rS1636-789 gene) of porcine epidemic diarrhea virus(PEDV) as well as preparation of its specific polyclonal antibodies. The amplified PEDV rS1636-789 gene was cloned into prokaryotic expression vector PGEX-6P-1, resulting in a recombinant plasmid PGEX- 6P- 1- rS1636- 789 followed by expression in E.coli BL21. The recombinant protein was expressed in the form of inclusion bodies, which was purified and used as immunogen to generate rabbit anti- rS1636- 789 polyclonal antibody. Indirect ELISA analysis showed that the titer of the polyclonal antibody was approximately 1 65 536. Western-blot showed that the polyclonal antibody could specifically bind to PEDV. The truncated PEDV epitope expressed in this study was a good candidate of PEDV engineering vaccines.