Puerarin Inhibits Inductible Nitric Oxide Synthase expression in Lipopolysaccharide-induced RAW264.7 Macrophage Cells

Objective To investigate the effect of Puerarin on expression of inductible nitric oxide synthase(iNOS)in RAW264.7 macrophage cells induced by lipopolysaccharide(LPS).MethodsThe protein and mRNA levels of lipopolysaccharide(LPS)-induced iNOS was determined in RAW264.7 macrophage cells using Western blotting and RT-PCR respectively.ResultsPuerarin inhibited expression of iNOS concentration dependently(P0.01).ConclusionPuerarin may reduce iNOS level,result in prevention of inflammation.