Experimental Research of Bushen Huoxue Herbs Combined with Staphylococcal Enterotoxin C Injection for Repairing Osteoporotic Fracture

Objective:This research was designed to evaluate the effect of Bushen Huoxue herbs combined with staphylococcal enterotoxin C injection(SECI) for repairing osteoporotic fracture(OPF),and to investigate the mechanism of action.Methods:80 female rabbits were chosen to make model of OP which were ovanriectomized,then their right radius were made closely fracture for OPF models and were randomized into 4 groups,20 rabbits in each group:group A(treated by Bushen Huoxue herbs combined with SECI),group B(injected local by SECI only),group C(treated by Bushen Huoxue herbs only),group D as the control group.The rabbits were sacrificed in the 4 different periods(2th week,4th week,6th week,8th week),and their right radius were taken for radiography,osteotylus anylysis by CT,hematoxylin and eosin(HE),FN,the immunohistochemistry index proliferating cell nuclear antigen(PCNA).All the date was calculated and statistically analyzed.Results:X ray observation showed:the healing in group A in different times was better than that in group B,C and D with the order of ACBD.CT test showed:the callus at different times in four groups had an increasing trend.At the 8th week,the CT result in group A was close to the normal level,with the order of ACBD.HE staining results showed:there were rich cells stained and ordered in group A at 2nd and 4th week,followed by group C and B.at 6th week,the callus in group A,B and C became mature and cartilage cells as well as osteoplast were reduced while osteoclast was increased.At 8th week,the moulding in group A appeared,followed by group B and C.In group D,there were still many cartilage cells and osteoplast.Immunohistochemical indicators showed that FN and PCNA were active in the early period of staining in group A,B and C and were higher than that in group D.As the healing development,FN and PCNA were reduced.At 8th week,there was no staining cells in group A,B and C.However,in group D,there was still a few expression.Through the whole process,the expression in group A at early stage was active and the reduce was also obvious.Conclusion:Bushen Huoxue herbs combined with staphylococcal enterotoxin C injection can improve the positive cell expressions of FN and PCNA in the early stage of osteoporotic fracture,enrich the quantity of callus,improve the trabecular bone structure and shorten the healing time,which has a better effect than single administration of Bushen Huoxue herbs or staphylococcal enterotoxin C injection.