末端脱氧核苷酸转移酶
微分脉冲伏安法
生物传感器
检出限
链霉亲和素
生物素
底漆(化妆品)
DNA
化学
核酸外切酶
生物物理学
DNA聚合酶
循环伏安法
生物化学
电化学
生物
电极
色谱法
标记法
有机化学
物理化学
细胞凋亡
作者
Xinyi Huang,Wuceng Niu,Jiayi Wu,Yan Wang,Chaorui Li,Jingfu Qiu,Jianjiang Xue
标识
DOI:10.1016/j.bios.2019.111609
摘要
In this research, a sensitive and specific electrochemical biosensor for DNA detection was constructed. The highly sensitivity of this biosensor is due to the exploitation of exonuclease III-assisted double recycling and toehold-mediated strand displacement recycling to achieve the target triple recycling amplification, thus generating a large amount of Y-shaped DNA structures. Combination with a terminal deoxynucleotidyl transferase (TDT)-mediated cascaded signal amplification strategy can catalyze the repetitive incorporation of biotin-dUTP to the 3'-OH of the Y-shaped DNA. Via biotin-streptavidin interaction, multiple streptavidin-alkaline phosphatases were conjugated to the surface of an Au electrode and generated a sharply increasing electrochemical signal in a 1-naphthyl phosphate (1-NP) solution. In this method, an impressive detection limit of 0.05 fM was obtained, presenting outstanding selectivity with a dynamic response scope between 0.1 fM and 1 nΜ. Thus, the designed biosensor opens an avenue for DNA detection in clinical molecular diagnostics, pathogen detection, gene therapy, food safety and environmental monitoring.
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