考马斯亮蓝
吸光度
布拉德福德蛋白质测定
色谱法
化学
双肌酸测定
生物化学
生物
染色
遗传学
作者
Clara L. Kielkopf,William R. Bauer,Ina L. Urbatsch
出处
期刊：CSH Protocols
[Cold Spring Harbor Laboratory Press]
日期：2020-04-01
卷期号：2020 (4): pdb.prot102269-pdb.prot102269
被引量：343
标识
DOI：10.1101/pdb.prot102269
摘要
The Bradford assay is a quick and fairly sensitive method for measuring the concentrations of proteins. It is based on the shift in absorbance maximum of Coomassie Brilliant Blue G-250 dye from 465 to 595 nm following binding to denatured proteins in solution.
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