Determination of physicochemical characteristics and molecular weight of bacteriocin 05-8 produced by Pediococcus acidilactici isolated from xinjiang koumiss.

This research focused on the physicochemical characteristics of bacteriocin 05-8 produced by Pediococcus acidilactici isolated from xinjiang koumiss. Antibacterial substance produced by Pediococcus acidilactici 05-8 was determined as bacteriocin using enzyme sensitivity experiment. It had heat stability up to 100 ℃ (90 min) and 121 ℃ (10 min). Antibacterial activity was remained during pH 2 to 8. It’s maximum production was 1280 AU during stationary phase and the best harvesting time was 13 h. It’s mode of action were bactericidal with Listeria monocytogenes as the indicator. Besides, It also exhibited the inhibition against other gram-positive bacteria, including Pediococcus pentosaceus, Streptococcus thermophilus, Enterococcus facealis etc. By Tricine-SDS-PAGE, it’s molecular weight was estimated to be belowe 14.4 kDa. These results provided the theory base for the use of the bacteriocin 05-8 in food preservation.