Fluorescent labeling of chitosan for use in non-invasive monitoring of degradation in tissue engineering

Journal of Tissue Engineering and Regenerative MedicineVolume 7, Issue 1 p. 39-50 Research Article Fluorescent labeling of chitosan for use in non-invasive monitoring of degradation in tissue engineering Cassilda Cunha-Reis, Cassilda Cunha-Reis Institute for Science and Technology in Medicine, School of Medicine, Keele University, Stoke-on-Trent, ST4 7QB UKSearch for more papers by this authorAlicia J. El Haj, Corresponding Author Alicia J. El Haj Institute for Science and Technology in Medicine, School of Medicine, Keele University, Stoke-on-Trent, ST4 7QB UKProfessor Alicia J. El Haj, Institute for Science and Technology in Medicine, School of Medicine, Keele University, Thornburrow Drive, Hartshill, Stoke-on-Trent, ST4 7QB, UK. E-mail: [email protected]Search for more papers by this authorXuebin Yang, Xuebin Yang Department of Oral biology, University of Leeds, Leeds, LS2 9 LU UKSearch for more papers by this authorYing Yang, Ying Yang Institute for Science and Technology in Medicine, School of Medicine, Keele University, Stoke-on-Trent, ST4 7QB UKSearch for more papers by this author Cassilda Cunha-Reis, Cassilda Cunha-Reis Institute for Science and Technology in Medicine, School of Medicine, Keele University, Stoke-on-Trent, ST4 7QB UKSearch for more papers by this authorAlicia J. El Haj, Corresponding Author Alicia J. El Haj Institute for Science and Technology in Medicine, School of Medicine, Keele University, Stoke-on-Trent, ST4 7QB UKProfessor Alicia J. El Haj, Institute for Science and Technology in Medicine, School of Medicine, Keele University, Thornburrow Drive, Hartshill, Stoke-on-Trent, ST4 7QB, UK. E-mail: [email protected]Search for more papers by this authorXuebin Yang, Xuebin Yang Department of Oral biology, University of Leeds, Leeds, LS2 9 LU UKSearch for more papers by this authorYing Yang, Ying Yang Institute for Science and Technology in Medicine, School of Medicine, Keele University, Stoke-on-Trent, ST4 7QB UKSearch for more papers by this author First published: 28 November 2011 https://doi.org/10.1002/term.494Citations: 34Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract The establishment of non-invasive analytical tools for assessing the in-situ use of biomaterials for surgical implants or scaffolds in tissue engineering and polymer-based therapies is fundamental. This study established a method for fluorescent tracking of the degradation of a chitosan membrane scaffold for use in vitro in bioreactors and ultimately in vivo. The basis of this tracking system is a fluorescence emitting biomaterial obtained by covalent binding of the fluorophore tetramethylrhodamine isothiocyanate (TRITC) onto the backbone of chitosan. Using confocal microscopy, this study quantitated the reductions in fluorescence intensity of the membrane and correlated these decreases with weight loss during polymer breakdown, thereby providing a technique for non-destructively assessing the extent of degradation of chitosan materials over time in vitro. Using multispectral imaging in a mouse model, the study assessed the degradation profile of the fluorophore-labeled biomaterial in vivo in real time and identified the dispersing pathway of the chitosan membrane degradation products in vivo. The results revealed that TRITC conjugated chitosan was biocompatible and supported bone cell growth. The changes in fluorescence intensity correlated well with weight loss up to 16 weeks of in vitro culture and could be monitored over two weeks in vivo. Copyright © 2011 John Wiley & Sons, Ltd. References Agnihotri SA, Kulkarni VD, Kulkarni AR, et al. 2006; Degradation of chitosan and chemically modified chitosan by viscosity measurements. J Appl Polym Sci 102: 3255– 3258. Bingaman S, Huxley VH, Rumbaut RE. 2003; Fluorescent dyes modify properties of proteins used in microvascular research. Microcirculation 10: 221– 231. 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