费斯特共振能量转移
化学
化学物理
偶极子
DNA
接受者
航程(航空)
分子物理学
结晶学
生物物理学
荧光
物理
凝聚态物理
光学
材料科学
复合材料
有机化学
生物
生物化学
作者
C. Steven Yun,Almudena Muñoz Javier,Travis L. Jennings,Matthew P. A. Fisher,Steven M. Hira,Stacey N. Peterson,Ben Hopkins,Norbert O. Reich,Geoffrey F. Strouse
摘要
Optical-based distance measurements are essential for tracking biomolecular conformational changes, drug discovery, and cell biology. Traditional Förster resonance energy transfer (FRET) is efficient for separation distances up to 100 Å. We report the first successful application of a dipole-surface type energy transfer from a molecular dipole to a nanometal surface that more than doubles the traditional Förster range (220 Å) and follows a 1/R4 distance dependence. We appended a 1.4 nm Au cluster to the 5‘ end of one DNA strand as the energy acceptor and a fluorescein (FAM) to the 5‘ end of the complementary strand as the energy donor. Analysis of the energy transfer on DNA lengths (15, 20, 30, 60bp), complemented by protein-induced DNA bending, provides the basis for fully mapping the extent of this dipole surface type mechanism over its entire usable range (50−250 Å). Further, protein function is fully compatible with these nanometal−DNA constructs. Significantly extending the range of optical based methods in molecular rulers is an important leap forward for biophysics.
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