群体感应
诱导剂
基因表达
细胞生物学
大肠杆菌
细胞
细胞生长
焊剂(冶金)
合成生物学
代谢途径
基因表达调控
化学
生物
基因
生物物理学
生物化学
计算生物学
毒力
有机化学
标识
DOI:10.1016/j.ymben.2015.04.005
摘要
Chemicals production by engineered microorganisms often requires induction of target gene expression at an appropriate cell density to reduce conflict with cell growth. The lux system in Vibrio fischeri is a well-characterized model for cell density-dependent regulation of gene expression termed quorum sensing (QS). However, there are currently no reports for application of the lux system to microbial chemical production. Here, we constructed a synthetic lux system as a tunable cell density sensor-regulator using a synthetic lux promoter and a positive feedback loop in Escherichia coli. In this system, self-induction of a target gene expression is driven by QS-signal, and its threshold cell density can be changed depending on the concentration of a chemical inducer. We demonstrate auto-redirection of metabolic flux from central metabolic pathways toward a synthetic isopropanol pathway at a desired cell density resulting in a significant increase in isopropanol production.
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