[Comparison of two capillary electrophoresis methods for aptamer-protein affinity characterization].

values from the NECEEM calculation approach by choosing 0.5-2-fold thrombin against F29-mer. This approach allowed the clear observation of an exponential bridge between the two peaks in the electrophoregrams. The CE-FA and PE-CZE methods could be used to mutually confirm each other, improving the affinity evaluation reliability. In this work, we recommend the selection of the CE-FA evaluation method as a priority, with a fitting mode through plateau peak height derived from a series of various concentrations. It could effectively address the problem of the high voltage-affected unstable complex peaks in CE. This provides such advantages that allow wide application, robust use, and feasible and accurate fitting results.