Preparation and Evaluation of a Novel 6-mono-Nitrophenylamino-β-cyclodextrin Bonded SBA-15 Chiral Stationary Phase for HPLC

A mono-nitrophenylamino-β-cyclodextrin was synthesized and bonded to the surface of ordered mesoporous SBA-15 for high performance liquid chromatography( HPLC),which obtained a novel 6-mono-nitrophenylamino-β-cyclodextrin bonded SBA-15 chiral stationary phase( NCDSP). Its structure and morphology were characterized by infrared spectroscopy,elemental analysis,thermal gravimetric analysis,mass spectrometry,scanning electron microscopy,transmission electron microscopy and X-ray diffraction. The basic chromatographic property of new NCDSP was evaluated and used for enantioseparations of chiral drugs such as β-blockers and dihydroflavanones. The fourteen kinds of β-blockers and six kinds of dihydroflavanones chiral drugs can be separated on NCDSP. The resolutions of metoprolol enantiomers and 2'-hydroxy flavanone enantiomers reached 1. 75 and 5. 21 within 20 min,respectively. By comparative study with PCDSP,some enantio-separation mechanisms of the new stationary phase were also discussed.