埃立克体
无浆体
生物
血红Rhipicephalus sanguineus
滴答声
兽医学
犬埃立克体
系统发育树
病毒学
硬蜱科
基因
遗传学
医学
血清学
抗体
作者
Ahmad Jafar Bekloo,Maryam Roya Ramzgouyan,Sadegh Shirian,Faezeh Faghihi,Hasan Bakhshi,Fatemeh Naseri,Mehdi Sedaghat,Zakkyeh Telmadarraiy
出处
期刊:Vector-borne and Zoonotic Diseases
[Mary Ann Liebert]
日期:2018-05-01
卷期号:18 (5): 252-257
被引量:10
标识
DOI:10.1089/vbz.2017.2219
摘要
Introduction:Anaplasma/Ehrlichia species are tick-transmitted pathogens that cause infections in humans and numerous domestic and wild animal species. There is no information available on the molecular characteristics and phylogenetic position of Anaplasma/Ehrlichia spp. isolated from tick species from different geographic locations in Iran. The aim of this study was to determine the prevalence, molecular characteristics, and phylogenetic relationship of both Anaplasma spp. and Ehrlichia spp. in tick species isolated from different domestic animals from two different geographical locations of Iran. Methods: A total of 930 ticks were collected from 93 cattle, 250 sheep, and 587 goats inhabiting the study areas. The collected ticks were then investigated for the presence of Anaplasma/Ehrlichia spp. using nested PCR based on the 16S rRNA gene, followed by sequencing. Sequence analysis was done based on the data published in the GenBank on Anaplasma/Ehrlichia spp. isolates using bioinformatic tools such as the standard nucleotide BLAST. Results: Genome of Anaplasma or Ehrlichia spp. was detected in 14 ticks collected in Heris, including 5 Dermacentor marginatus, 1 Haemaphysalis erinacei, 3 Hyalomma anatolicum, and 4 Rhipicephalus sanguineus, also in 29 ticks collected in Chabahar, including 14 R. sanguineus, 8 D. marginatus, 3 Hyalomma Anatolicum, and 4 Hyalomma dromedarii. Partial analysis of the 16S rRNA gene sequence of positive samples collected from goats and sheep showed that they were infected with Anaplasma/Ehrlichia spp. that were 94–98% identical to ovine Anaplasma and 91–96% identical to Neoehrlichia and Ehrlichia spp. Conclusion: The various ticks identified in this study suggest the possible emergence of tick-borne diseases in animals and humans in these regions. R. sanguineus and D. marginatus seem to be predominant vectors responsible for anaplasmosis in these regions. Partial sequence analysis of the 16S rRNA gene showed that A. ovis is genetically polymorphic in these regions. Furthermore, an association between the genetic heterogeneity of this microorganism and the geographical regions of Anaplasma strains was found. This study also showed that those ticks that were collected from the same geographical origin were infected with closely related strains of Anaplasma.
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