基因簇
真菌
体外
细胞毒性
消炎药
一氧化氮
脂多糖
曲霉
微生物学
化学
海绵
基因
生物
生物化学
立体化学
植物
药理学
有机化学
免疫学
作者
Lekshmi Niveditha,Peng Fu,Tiago Leão,Te Li,Tingting Wang,Remington X. Poulin,Lorena Rigo Gaspar,C. Benjamin Naman,Sajeevan Thavarool Puthiyedathu
出处
期刊:Planta Medica
[Georg Thieme Verlag KG]
日期:2022-02-11
卷期号:88 (09/10): 774-782
被引量:2
摘要
In following up on observed in vitro anti-inflammatory activity of the organic extract of the marine sponge-derived fungus Aspergillus tamarii MCCF102, two new dipyrrolobenzoquinones, terreusinone B and C (1: and 2: ), were discovered along with the known analogue, terreusinone (3: ). The structures of 1: -3: were determined by spectroscopic and spectrometric analyses, along with chemical inter-conversion. In vitro testing on lipopolysaccharide (LPS) stimulated RAW 264.7 murine macrophage cells revealed that 1: -3: exhibit anti-inflammatory activity by inhibiting nitric oxide production in a dose-dependent manner (IC50 < 1 µM) without any cytotoxicity observed at the same concentrations. Due to this and the UV-A absorptive properties imparted by the highly conjugated structures of these molecules, the potential for using 1: -3: or related analogues as natural sunscreen components is suggested. Gene sequencing and informatics biosynthetic gene cluster comparisons were insufficient to confidently elucidate the biosynthetic origins of these compounds, possibly suggesting the occurrence of a gene cluster not detected in the initial sequencing or a non-canonical pathway that should be further investigated.
科研通智能强力驱动
Strongly Powered by AbleSci AI