Tetracycline biotransformation by a novel bacterial strain Alcaligenes sp. T17

Comprehensive knowledge on the biotransformation of tetracycline (TC) is critical for the improvement of TC removal in the bioremediation process. This work isolated a novel TC-degrading bacterial strain Alcaligenes sp. T17 and explored its degradation ability under different conditions. Temperature and pH could affect the degradation efficiency, and higher temperature as well as neutral and weakly acidic conditions were conducive to the biotransformation. Response surface methodology predicted the maximum degradation rate of TC (94.35%) under the condition of 25.15 mg/L TC, pH 7.23, and inoculation dosage 1.17% at 40 °C. According to the result of disk diffusion tests, the biodegradation products had lower antimicrobial potency than the parent compound. Five potential biodegradation products were identified, and a possible degradation pathway (degrouping, oxidation and ring-opening) was proposed. The draft genome of strain T17 was also determined. Genomic analysis indicated that strain T17 harbored multiple genes that participated in the metabolism of aromatic compounds as well as genes encoding oxygenases. These functional genes may be relevant to TC biotransformation. This study could provide new insights towards the biotransformation of TC mediated by bacteria. • An Alcaligenes strain capable of degrading tetracycline was isolated from sediment. • Higher temperature and neutral and weakly acidic condition favored tetracycline degradation. • Biotransformation products had lower antimicrobial potency than parent compound. • TC-degrading pathways were proposed and possible genes involved in TC degradation were explored.