免疫分析
纳米花
化学
纳米技术
检出限
尿素酶
组合化学
一级和二级抗体
材料科学
色谱法
纳米结构
抗体
酶
生物化学
生物
免疫学
作者
Hongxia Li,Xu Yan,Deshuai Kong,Dandan Su,Fangmeng Liu,Peng Sun,Xiaomin Liu,Chenguang Wang,Xiaoteng Jia,Geyu Lu
标识
DOI:10.1016/j.bios.2022.114132
摘要
Fundamentally improving the sensing sensitivity of immunoassay remains a huge challenge, which limited further critical applications. Herein we designed a new immunoprobe by integrating biometric unit (antibody) and signal amplification element (enzyme) to form urease-antibody-CaHPO4 hybrid nanoflower (UAhNF) via the biomineralization process. The dual-functional UAhNF enhances the stability of urease in NaCl (10 mmol L-1) and high temperature (60 °C), and also maintains the ability of antibody recognition, fitting greatly well with the need for immunosensor. Using imidacloprid as a model target, the fixed coating antigens are competed with imidacloprid to capture primary antibodies, and the secondary antibody of UAhNF was linked to construct the competitive-type fluorogenic immunoassays. An in-situ etching process of copper nanoparticles initiated by urease is integrated with UAhNF-based immune response for further improving the detection sensitivity. The proposed immunosensor possessed a 50% inhibition concentration value of 0.72 ng mL-1, which is 30-fold lower than conventional enzyme-linked immunosorbent assay. This presented approach provided a versatile sensing tool by varying building blocks, making it practically functional for a variety of bioassay applications.
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