PENGARUH VAKSINASI OUTER MEMBRAN PROTEIN 52 kDa Aeromonas hydrophila TERHADAP PENINGKATAN JUMLAH DAN HITUNG JENIS LEKOSIT IKAN NILA (Oreochromis niloticus) YANG DIINFEKSIAeromonas hydrophila

Nile Tilapia Fish (Oreochromis niloticus) is a freshwater fish that grows fast and easy to be cultured. One of the difficulties in culturing nile tilapia fish is the infection of Aeromonas hydrophila that causing Motil Aeromonas Septicemia (MAS) disease. This study aimed to know the increasing of the total and differential counting of nile tilapia fish leukocytes after being vaccinated by outer membrane protein 52 kDa Aeromonas hydrophila for 1 week and infected with Aeromonas hydrophila CFU/ml for 4 days. This study used 20 Nile Tilapia fish with 10-12 cm in length and divided into 4 treatments and 5 repetitions which are P0(-) (not vaccinated and not infected), P0(+) (not vaccinated and infected), P1 (vaccinated with whole cell protein HydroVac® and infected), P2 (vaccinated with outer membrane protein 52 kDa and infected) by intramuscular injection. The data analysis of this research used Analysis of Variants (ANOVA). If there is a difference among treatments it will be continued to Duncan's Range Multiple Test with a significance level of 5%. The post-infection observation results in P2 showed there was significant increase of the total of leukocytes and lymphocytes.