组蛋白
染色质免疫沉淀
赖氨酸
组蛋白H2A
组蛋白密码
生物化学
组蛋白H3
生物
乙酰化
细胞生物学
化学
计算生物学
表观遗传学
DNA
核小体
基因
基因表达
氨基酸
发起人
作者
Xiaozhe Xiong,Tatyana Panchenko,Shuang Yang,Shuai Zhao,Peiqiang Yan,Wenhao Zhang,Wei Xie,Yuanyuan Li,Yingming Zhao,C. David Allis,Haitao Li
标识
DOI:10.1038/nchembio.2218
摘要
Recognition of histone covalent modifications by 'reader' modules constitutes a major mechanism for epigenetic regulation. A recent upsurge of newly discovered histone lysine acylations, such as crotonylation (Kcr), butyrylation (Kbu), and propionylation (Kpr), greatly expands the coding potential of histone lysine modifications. Here we demonstrate that the histone acetylation-binding double PHD finger (DPF) domains of human MOZ (also known as KAT6A) and DPF2 (also known as BAF45d) accommodate a wide range of histone lysine acylations with the strongest preference for Kcr. Crystal structures of the DPF domain of MOZ in complex with H3K14cr, H3K14bu, and H3K14pr peptides reveal that these non-acetyl acylations are anchored in a hydrophobic 'dead-end' pocket with selectivity for crotonylation arising from intimate encapsulation and an amide-sensing hydrogen bonding network. Immunofluorescence and chromatin immunoprecipitation (ChIP)-quantitative PCR (qPCR) showed that MOZ and H3K14cr colocalize in a DPF-dependent manner. Our studies call attention to a new regulatory mechanism centered on histone crotonylation readout by DPF family members.
科研通智能强力驱动
Strongly Powered by AbleSci AI