外体
微泡
下调和上调
细胞生物学
转染
心肌梗塞
细胞生长
内吞作用
细胞
再生(生物学)
细胞周期
小RNA
医学
癌症研究
生物
心脏病学
细胞培养
基因
遗传学
生物化学
作者
Yuxin Wang,Ning Ding,Ge Guan,Ge Liu,Da Huo,Yanzhao Li,Keyu Wei,Jingyuan Yang,Panke Cheng,Chuhong Zhu
标识
DOI:10.1166/jbn.2018.2493
摘要
Acute myocardial infarction leads to heart failure due to inadequate regeneration of cardiomyocytes. Therefore, promotion of cardiomyocyte proliferation is the key for the restoration of cardiac function. Induction of the cell cycle and the downregulation of genes that inhibit cardiomyocyte proliferation could induce cardiomyocyte to re-enter into the proliferative state. Hsa-miR-590-3p has good application prospects in myocardial proliferation since it could downregulate the expression of genes inhibiting cell proliferation such as Hopx. However, delivering sufficient hsa-miR-590-3p to the infarct area with non-invasive and non-viral methods efficiently and rapidly is challenging. Based on the high expression of cTnI in the microenvironment of infarct area, we used gene transfection to express a cTnI-targeted short peptide on the surface of mesenchymal stem cells to obtain cTnI-targeted exosomes. These exosomes could localize to infarct area along a cTnI concentration gradient. Exosomes carrying hsa-miR-590-3p were endocytosis by cardiomyocytes and thus promoted cardiomyocyte proliferation in the peri-infarct area and eventually restored cardiac function. Our results show that targeted exosome is a minimally invasive, non-viral, efficient, and rapid delivery system for the treatment of acute myocardial infarction.
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