[Object] To analyze the gastrodin content in wild and cultivated Gastrodia elata Bl.from Tibet.[Method] The gastrodin content in Gastrodia elata Bl.was determined by HPLC in this study.[Result] The content of gastrodin in cultivated Gastrodia elata was in the range of 0.41-2.28 g/kg,and the average content was 0.88 g/kg,4 times higher than the wild ones.[Conclusion] Based on the similar ecological environment,growth period and harvest season,the quality of cultivated Gastrodia elata was superior to that of wild ones.Popularizing cultivation technology of Gastrodia elata is of great practical significance and high economic value.