壳聚糖
涂层
骨整合
骨形态发生蛋白
骨形态发生蛋白2
粘附
生物医学工程
材料科学
化学
化学工程
生物物理学
纳米技术
植入
复合材料
生物化学
体外
医学
外科
生物
基因
工程类
作者
Lu Chen,Jun Lin,Juan Li,Xiaozhao Wang,Jingjing Zhuang,Huiming Wang,Kui Cheng,Wenjian Weng
标识
DOI:10.1016/j.colsurfb.2016.04.047
摘要
In this study, mineralized collagen (COL) coatings with controlled loading and release of bone morphogenetic protein (rhBMP-2) as well as enhanced osteogenic differentiation were successfully achieved via the spatially-control of hydroxypropyltrimethyl ammonium chloride chitosan (HACC) within the coatings. The distribution of HACC in the inner part (HACC-IN) or the outer part (HACC-OUT) of the coatings were adjusted by different potential values and negative/positive alternations during alternating potentials assisted electrochemical deposition (AP-ECD). It was found that rhBMP-2 loading capacity was remarkably enhanced with the increased incorporation of HACC due to their strong interaction, and the release behavior was also tuned by HACC location. In general, HACC-IN coatings showed a prominent improvement in cytocompatibility and osteogenic differentiation. The main reason is considered that the inner location of HACC can eliminate the negative effect of HACC to initial cellular adhesion and bring to a sustained rhBMP-2 release behavior due to kinetic modification. An optimized coating in this work could load as high as 4644 ng/cm2 rhBMP-2 and release only 25% for 14 days, which consequently leads to a better osteogenic differentiation. This study has thus inspired another promising protocol for designing growth factor incorporated bioactive coatings for bone implants with improved osteointegration.
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