CLINICAL APPLICATION OF INTRACYTOPLASMIC SPERM INJECTION FOR MALE INFERTILITY CASES

Objective To apply the technique of intracytoplasmic sperm injection (ICSI) to male infertility resulting from severe oligozoospermia, asthenozoospermia and obstructive azoospemia. Methods After genetic counseling and chromsome ana- lysis, 28 couples were accepted. Occytes were collected from wives with controlled ovarian stimulation. Sperm from husbands with oligozoospermia or asthenozoospermia by density-gradient centrifugation, or from whom with obstructive azoospermia by epididymal sperm aspiration or testicular sperm aspiration . Mature oocytes were fertilized by ICSI. Results 361 oocytes were collected in 32 cycles. Among which 306 were mature. 256 were fertilized after ICSI. The fertilization rate was 83.7%. 233 oocytes developed to the stage of cleavage. The cleavage rate was 91%. Clinical pregnancy was achieved in 11 cycles. The pregnancy rate was 34.4%. Conclusion The technique of ICSI is an efficient method for the male infertility therapy.