DE7,a bacterial strain that can effectively degrades 17α-ethynylestradiol(EE2),was isolated from activated sludge of wastewater treatment plant.Based on its physiological and biochemical characters,as well as 16S rDNA sequence analysis,this strain was identified as Cupriavidus.The optimal temperature and pH for the strain utilizing EE2 was 30℃ and 7,respectively.Under the degradation of EE2 with initial concentration of 25 mg·L-1,the 82% degradetion rate was obtained indicated that the degradation rate of EE2 by the strain within 7 days was 82%.