Effect of transforming growth factor β1 on proliferation of JEG-3 choriocarcinoma cells and Smad 3,7 mRNA expression

Objective:To observe the proliferation of JEG-3 choriocarcinoma cells and Smad 3,7 mRNA expression changes under the action of transforming growth factor β1(TGF-β1),explore the mechanism of TGF-β/Smads signal channel in occurrence and development of choriocarcinoma.Methods:The JEG-3 choriocarcinoma cells were treated with different concentrations and times of TGF-β1,the cell proliferation activity was detected by MTT method,the difference of Smad 3,7 mRNA expression was detected by RT-PCR.Results:TGF-β1 stimulated the proliferation of JEG-3 choriocarcinoma cell;the expression level of Smad 3 mRNA gradually increased with the increase of TGF-β1 concentration;after treated with a certain concentration of TGF-β1 for 12 hours,the expression level of Smad 3 mRNA gradually increased with time passing;under the same condition,there was no significant difference in expression level of Smad 7.Conclusion:TGF-β1 can stimulates the proliferation of JEG-3 choriocarcinoma cell;the reaction of Smad 3 mRNA to exogenous TGF-β1 is good,while Smad 7 mRNA has no response.