Influence of Different Sampling Ways on Blood Cell Parameters in Rat

The objective of the study is to discuss the differences of blood cell parameters in rat by different sampling ways. Blood cells collected from abdominal aorta, orbitalis vein, jugular aorta and tail vein of 46 healthy rats were detected by ABBOTT CD-3700sl Automatic Hematology Analyzer. The parameters of WBC, RBC, Hb, HCT, MCV, MCH, MCHC and PLT from different sampling ways were analyzed and compared with those from abdominal aorta using paired t test. The results were as follows: all the parameters in tail vein group and orbital vein group were significantly higher than those in abdominal aorta group ( P 0.01), except MCV. While only the WBC in jugular aorta group was different from that in abdominal aorta group ( P 0.05). As to complete blood cell, orbital vein and tail vein were significantly different from abdominal aorta, but the difference was minor between jugular aorta and abdominal aorta. In a word, blood sampling ways would have effect on CBC. So each laboratory should set up own data base to construct consistent background.