Simultaneous Determination of Qunolones, Sulfonamides and Tetracyclines Residues in Eel Using Solid Phase Extraction (SPE) and Ultra-performance Liquid Chromatography-Electrospray Tandem Mass Spectrometry (UPLC-MS-MS)

A robust and sensitive UPLC-MS-MS method was developed for the simultaneous determination of residues of 15 sulfonamides, 3 fluoroquinolones and 4 tetracylines in eel. The target compounds were extracted with acidic EDTA-Mcllvaine buffer solution, and enriched and cleared up using a hydrophilic-lipophilic balance (HLB) SPE cartridge. After seperation on UPLC BEH C18, the analytes were detected using a tandem mass spectrometry and quantified in multiple reaction monitoring (MRM) mode. At the spike levels of 5, 10 and 20 μg/kg respectively, the average recoveries for all the compounds were 72.4%-97.3%, and the variation coefficients were all under 14.4%. This method is simple, fast, sensitive, and particular for the simulaneous determination of sulfonamides, fluoroquinolones and tetracylines in eel.