The Expression Level of miRNAs in Pancreatic Cancer Cell Lines and Pancreatic Cancer Tissues

Objective: To investigate the relative abundances of those miRNAs screened by microarray in pancreatic cancer cell lines and in pancreatic cancer tissues and confirm the correlations between those miRNAs and pancreatic cancer.Methods: The total RNA were extracted from pancreatic cancer cell lines and pancreatic tissue.The 17 miRNAs(miR-16,miR-20a,miR-21,miR-26a,miR-142-3p,miR-155,miR-210,miR-181a,miR-181b,miR-196a,miR-939,miR-223,miR-1202,miR-1207-5p,miR-1825,miR-1228,miR-1915) were detected by Real-time PCR.And then we can analyze the relationship between miRNAs and clinical characteristics of patients with pancreatic cancer.Results: Compared with normal pancreatic tissue,the expression of miRNAs in pancreatic cancer cell lines and the pancreatic cancer tissue were significantly increased.Amount of miRNAs expression in carcinoma tissues and adjacent tissues in patients with pancreatic cancer has no statistical difference in different genders,and it has little correlation with the age and the level of serum CA19-9.Conclusion: The screened miRNAs can be as potential diagnostic markers of pancreatic cancer.MiR-223 expression level was negatively correlated with the maximum tumor diameter and tumor stage,which suggested that miRNAs may change with the occurrence and progress of pancreatic cancer.