Introduced the basic characteristics of pine mushroom (tricholoma matsutake) and its physiological function Although pine mushroom is rich of nutrition, wild pine mushroom cannot be cultured artificially A great number of pine mushroom mycelium can be obtained by liquid deep culture technology Introduced emphatically the culture methods of pine mushroom mycelium, the culture isolation process and fermentation conditions Pine mushroom polysaccharide which included endocellular polysaccharide and extra cellular polysaccharide was extracted preliminarily under liquid deep culture condition and determined the proportion between endocellular and extra cellular polysaccharide.