A study on the Morphology of Trigeminal Ganglion in Mice by Ophthalmic Nerve Injected with DiI

Objective: To investigate the morphology of the trigeminal ganglion in mice by ophthalmic nerve injection with DiI.Methods: This experiment with 10 mice,body weight 25-30g,Perfusion fixed,then the trigeminal ganglion and ophthalmic nerve were exposed under surgical microscope and Dil was embedded into ophthalmic nerve.The tissue were incubated at 37℃ for 3 months and sectioned,mounted,then the location and morphology of trigeminal ganglion in mice was observed under a fluorescence microscope after satisfactorily diffused with Dil.Rusults: After Dil was embedded into ophthalmic nerve,the labeled cells in ophthalmic nerve fibers passed through the the superior orbital fissure into the trigeminal ganglion,the labeled ganglion cells located in Anterior medial part of trigeminal ganglion.The DiI labeled ganglion cell and nerve fiberS can be observed in the trigeminal ganglion.The nerve fibers separated in groups or clusters by ganglion cells.The labeled nerve fibers were parallel dense,the nerve cells were round and oval with different size,the neurite can be observed and connected to the cell body spirally and linearly,then formation the nerve terminals and synaptic connections and bipolar neurons can be observed.Conclusion: The trigeminal ganglion cells and nerve fibers in mice consistent with other animals after ophthalmic nerve injection with DiI.