Isolation and Identification of a Thermophile Bacterium and Cloning of Trehalose Synthase Gene

A thermophile strain,designated as TTH,was isolated from 80 ℃ hot spring at Hezhou county in Guangxi province.It could grow at temperatures from 55 ℃ to 85 ℃,with the optimal temperature 70℃,the highest temperature 85℃,and with 67.6% molar content of the G+C.The researches which were based on the TTH morphological,culture conditions,16S rDNA sequence,as well as the phylogenetic analysis show that the strain TTH has 98% sequence identity with three kinds of Thermus thermophilus.Therefore the strain is tentatively identified as Thermus thermophilus.Trehalose synthase gene of TTH was cloned and analyzed.It has 87% identity to the treS gene from Thermus thermophilus RQ-1,Thermus caldophilus,Thermus thermophilus HB8 and Thermus thermophilus.