Effect of donepezil against acetylcholinesterase activity in brain and blood

Objective To investigate the changes of acetylcholinesterase(AChE) activity in brain,blood and plasma after single dose administration of donepezil.Methods Sixty-four mice were divided into eight groups: normal control(0.9 % NaCl,0.2 mL /10 g,test at 2 h) and donepezil(0.625 mg/kg;test at 0.5,1,2,4,8,12 and 24 h,respectively) groups.Blood and brain assays were collected at the fixed-point of time after oral administration.The AChE activity and the content of protein were measured by AChE test kits and protein test kits respectively.Results The AChE activity in brain of 2 h group showed significant decreased(P0.05).Others showed no difference(P0.05).Conclusion Single dose(0.625 mg/kg) of donepezil can reduce AChE activity in brain after oral administration 2 h,but not in blood and plasma.