Transfection of Foreign DNA into NIH3T3 Cell Mediated by Cationic Liposome

Objective To find a high explore by efficient method of transfecting foreign DNA into NIH3T3 cells by cationic liposome. Methods With green fluorescence protein(GFP) as report gene,transfection efficiency was compared by LipofectAMINE2000,GenePORTs and LipofectAMINE in NIH3T3 cell using FACS,and the transfection condition was optimized in cell confluence,liposome quantity,liposome/DNA ratio and transfecting time. Results Accor-(ding) to their transfecting efficiency,the three liposomes were ranked degressively: LipofectAMINE2000,GenePORTs and LipofectAMINE.The highest transfecting efficiency of LipofectAMINE2000 would be obtained when cell confluence was 60%~70%,liposome concentration 3 μL/1×10~(6)cells,ratio of liposome/DNA 3 μL/1μg(and transfecting) time was 5 hours.The cells could express GFP more than one month. Conclusion LipofectAMINE2000 is the best liposome and its transfection condition would be optimized.