卟啉
荧光
水溶液
化学
衍生工具(金融)
水溶性
光化学
色谱法
有机化学
光学
金融经济学
物理
经济
作者
Jiajun Chen,Chenyang Zhang,Weiye Nie,Lidan Liu,Qi Zhou,Wenwu Cao
出处
期刊:Analytical Methods
[The Royal Society of Chemistry]
日期:2025-01-01
卷期号:17 (21): 4351-4358
被引量:2
摘要
Accurately tracing copper ions (Cu2+) is critically important in the fields of environmental protection and human body monitoring. Excessive intake of Cu2+ not only easily leads to diseases, but also affects human health. Therefore, it is particularly important to find an easy-to-use, effective, and highly selective probe for in situ Cu2+ detection in living cells. Herein, a water-soluble porphyrin derivative (WSPD) was successfully synthesized and used as a highly sensitive and selective fluorescent probe for detecting Cu2+ in aqueous solutions and living cells. The WSPD possesses good water solubility and photostability, large Stokes shift (>200 nm), appreciable red fluorescence and low cytotoxicity. The fluorescence signal could be rapidly quenched by Cu2+ with high selectivity due to the strong affinity of Cu2+ to the porphyrin core of the WSPD. The proposed fluorescent probe exhibited a low detection limit of 6.43 nM and a fast response time (<5 min) for Cu2+ detection in aqueous solution. Furthermore, it is successfully employed for the fluorescence imaging of intracellular Cu2+ in living cells, demonstrating its great potential in the fields of biomedical imaging and biosensing.
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