Effects of Short-Term Temperature Stress on Metabolic and Digestive Enzymes Activities of Procambarus clarkii

To investigate the effects of temperature stress on metabolic and digestive enzyme activities of Procambarus clarkii , the test species was transferred from 20°C (control group) to 10°C and 30°C, and samples were collected at 0, 3, 6, 12, 24, and 48h. The activities of pyruvate kinase (PK), hexokinase (HK) and hosphoenolpyruvate carboxykinase (PEPCK) in muscle and hepatopancreas of Procambarus clarkii was determined. The changes in α-amylase (α-AMY) and lipase (LPS) activity in intestinal and hepatopancreas were also examined. The results showed that the activities of digestive and metabolic enzymes in Procambarus clarkii were significantly affected by extreme water temperature ( P<0.05 ). During the whole experimentation period, PK, HK, and PEPCK activities in the low-temperature group (10°C) were lower than those in the control group (20°C) ( P < 0.05 ). Meanwhile, LPS and α-AMY activities in the low-temperature group (10°C) were also significantly lower than those in the control group (20°C) ( P < 0.05 ). In the high-temperature group (30°C), the activities of PK, HK, and PEPCK metabolic enzymes in muscle and hepatopancreas decreased first. Then they increased, and their activity levels were significantly lower than those in the control group ( P<0.05 ). At the same time, the activities of LPS and α-AMY digestive enzymes in the intestines and hepatopancreas were significantly lower than those in the control group( P<0.05 ). In summary, this study examined the impact of temperature stress on the metabolic and digestive enzyme activities in Procambarus clarkii , shedding light on its self-regulation mechanisms in response to temperature fluctuations. The findings provide a scientific foundation for understanding the species’ adaptation to environmental changes.