PmRGL2/PmFRL3–PmSVP Module Regulates Flowering Time in Japanese apricot (Prunus mume Sieb. et Zucc.)

Temperate fruit trees rely on environmental and endogenous signals to trigger dormancy release and flowering. However, the knowledge of DELLA protein PmRGL2, a Prunus mume homolog of REPRESSOR OF GA-Like 2 (RGL2), which serves as an important inhibitory factor in gibberellin (gibberellin acid [GA]) signalling, is limited related to on its regulatory effects on dormancy release and flowering. In our study, the protein-protein interaction assays showed an interaction between PmRGL2 and PmFRL3, a Prunus mume homolog of FRIGIDA-LIKE (FRL). The FRL protein regulates flowering induction by binding to chaperone proteins. To understand the transcriptional regulation of PmRGL2 in Prunus mume, in detail's we constructed a ChIP-Seq library at four key stages of flower bud development. Genome-wide analysis screened a MCM1-AGAMOUSDEFICIENS Serum Response Factor box (MADS box) protein for two SHORT VEGETATIVE PHASEs (SVPs). Genetic analysis showed that overexpressing PmSVP in Arabidopsis thaliana reduced the GA content and delayed flowering, whereas PmSVP-like overexpression increased the GA content and promoted flowering. Protein-DNA binding assays revealed that the PmRGL2/PmFRL3 protein complex promoted PmSVP transcription while repressing PmSVP-like transcription, which inhibited the flowering process. As chilling requirements increased, the PmFRL3 protein was degraded. ThePmRGL2/PmFRL3 protein complex is disrupted. With the increase in the GA content within the flower buds, the PmRGL2 protein was degraded in response to GA signalling, and the function of PmSVP-like was released. It dominated flowering, leading to this process in Prunus mume. Therefore, we propose a mechanism by which the PmRGL2/PmFRL3 protein complex responds to GA and low-temperature signalling to regulate PmSVP and PmSVP-like synergistically and thus Prunus mume flowering time.