The contamination of food by spoilage microorganisms is a major concern for public health. Food-borne pathogens are commonly encapsulated by biofilms. Bacteriocins of probiotic lactic acid bacteria, such as nisin and pediocin have been used as bio-preservatives to improve food safety and extend the storage period of some food types. Therefore, there is a need to explore the potential of bacteriocins to impede the growth and biofilm formation by food spoilage bacteria in milk.