代谢工程
2,3-丁二醇
大肠杆菌
代谢物
化学
生物化学
醇脱氢酶
糖酵解
焊剂(冶金)
丙酮酸脱氢酶复合物
新陈代谢
酶
发酵
有机化学
基因
作者
Tayyab Islam,Thuan Phu Nguyen-Vo,Seung-Hyun Cho,Junhak Lee,Vivek Kumar Gaur,Sunghoon Park
标识
DOI:10.1016/j.biortech.2023.129814
摘要
1,3-Butanediol (1,3-BDO) finds versatile applications in the cosmetic, chemical, and food industries. This study focuses on the metabolic engineering of Escherichia coli K12 to achieve efficient production of 1,3-BDO from glucose via acetoacetyl-CoA, 3-hydroxybutyryl-CoA, and 3-hydroxybutyraldehyde. The accumulation of an intermediary metabolite (pyruvate) and a byproduct (3-hydroxybutyric acid) was reduced by disruption of the negative transcription factor (PdhR) for pyruvate dehydrogenase complex (PDHc) and employing an efficient alcohol dehydrogenase (YjgB), respectively. Additionally, to improve NADPH availability, carbon flux was redirected from the Embden-Meyerhof-Parnas (EMP) pathway to the Entner-Doudoroff (ED) pathway. One resulting strain achieved a record-high titer of 790 mM (∼71.1 g/L) with a yield of 0.65 mol/mol for optically pure (R)-1,3-BDO, with an enantiomeric excess (e.e.) value of 98.5 %. These findings are useful in the commercial production of 1,3-BDO and provide valuable insights into the development of an efficient cell factory for other acetyl-CoA derivatives.
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