生物传感器
金黄色葡萄球菌
荧光
比色法
双模
化学
线性范围
适体
锰
纳米技术
材料科学
色谱法
检出限
光学
细菌
有机化学
物理
工程类
航空航天工程
生物
遗传学
作者
Xue Gao,Hongmei Zhang,Linfeng Lu,Mu Jia,Xuepeng Li,Jiànróng Lǐ
出处
期刊:Food Chemistry
[Elsevier]
日期:2024-01-01
卷期号:432: 137144-137144
被引量:5
标识
DOI:10.1016/j.foodchem.2023.137144
摘要
A ratiometric fluorescence and colorimetry dual-mode nano-biosensor has been established for Staphylococcus aureus (S. aureus) determination. The prepared approaches of Manganese dioxide nanosheets (MnO2 NSs) and carbon dots (BCDs) were facile, efficient and labor-saving and MnO2 NSs-mediated fluorescence quenching and oxidation could amplify detection signals. The dual-mode determination had a broad linear range of 37 ∼ 3.7 × 106 CFU/mL and low detection limits of 9 CFU/mL (ratiometric fluorescence) and 22 CFU/mL (colorimetry). Meanwhile, the method was applied in real samples with recovery ranging of 90 ∼ 102% and RSD < 4.44%, which was an insignificant difference with standard plate counting. The new dual-mode approach of S. aureus possesses the advantages of superior sensitivity, precision, accuracy and specificity. Moreover, the dual-mode nano-biosensor can be adopted in other foodborne pathogens determination by changing corresponding aptamers and provide an enlightenment in monitoring food safety.
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