适体
恩诺沙星
指数富集配体系统进化
检出限
线性范围
石墨烯
色谱法
材料科学
生物
化学
纳米技术
分子生物学
生物化学
抗生素
核糖核酸
环丙沙星
基因
作者
Xiaoyi Bao,Siyuan Wang,Qingfang Hao,Yuhang Bai,Siying Li,Shuai Zhang,Lei Zhang,Xiaoning Kang,Mingsheng Lyu,Shujun Wang
出处
期刊:Foods
[MDPI AG]
日期:2024-03-20
卷期号:13 (6): 941-941
标识
DOI:10.3390/foods13060941
摘要
Enrofloxacin (ENR) is widely used as a synthetic fluoroquinolone antibiotic for disease control in aquatic animals. ENR aptamers were screened in this study using the magnetic bead-SELEX method, and a graphene oxide fluorescent sensor was developed to detect the ENR residues in aquatic products. Firstly, ENR was conjugated to amino magnetic beads by amidation reaction, and then the aptamer sequences showing high affinity to ENR were screened step by step by using the SELEX screening method. Finally, after 10 rounds of SELEX screening, six candidate aptamers with high affinity were obtained. Among these, ENR-Apt 6 was selected based on its secondary structure features, high affinity (Kd = 35.08 nM), and high specificity to ENR. Furthermore, a fluorescent sensor was prepared using graphene oxide and ENR-Apt 6. The results showed that the linear range of the sensor could reach 600 nM (R2 = 0.986), while its optimal linear range was 1-400 nM (R2 = 0.991), with the lowest detection limit of 14.72 nM. The prepared sensor was successfully used for the detection of ENR in real samples, with a recovery range of 83.676-114.992% and a relative standard deviation < 10% for most of the samples.
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