Glucose Oxidase Energized Osmium with Dual‐Active Centers and Triple Enzyme Activities for Infected Diabetic Wound Management

Abstract Diabetic wounds are susceptible to bacterial infections, largely linked to high blood glucose levels (hyperglycemia). To treat such wounds, enzymes like glucose oxidase (GOx) can be combined with nanozymes (nanomaterials mimic enzymes) to use glucose effectively for purposes. However, there is still room for improvement in these systems, particularly in terms of process simplification, enzyme activity regulation, and treatment effects. Herein, the approach utilizes GOx to directly facilitate the biomineralized growth of osmium (Os) nanozyme (GOx‐OsNCs), leading to dual‐active centers and remarkable triple enzyme activities. Initially, GOx‐OsNCs use vicinal dual‐active centers, enabling a self‐cascaded mechanism that significantly enhances glucose sensing performance compared to step‐by‐step reactions, surpassing the capabilities of other metal sources such as gold and platinum. In addition, GOx‐OsNCs are integrated into a glucose‐sensing gel, enabling instantaneous visual feedback. In the treatment of infected diabetic wounds, GOx‐OsNCs exhibit multifaceted benefits by lowering blood glucose levels and exhibiting antibacterial properties through the generation of hydroxyl free radicals, thereby expediting healing by fostering a favorable microenvironment. Furthermore, the catalase‐like activity of GOx‐OsNCs aids in reducing oxidative stress, inflammation, and hypoxia, culminating in improved healing outcomes. Overall, this synergistic enzyme‐nanozyme blend is user‐friendly and holds considerable promise for diverse applications.