Transcardial perfusion of mouse tissues v1

This protocol describes how to perform transcardial perfusion and fixation of mouse brain tissues in preparation for immunohistochemical staining or histology. This process includes lethal overdose of mice with sodium pentobarbitone, occlusion of the descending aorta, followed by transcardial perfusion with phosphate buffered saline to clear blood from the vasculature and paraformaldehyde to fix mouse brain tissues.